FastQCFastQC Report
Thu 27 Aug 2020
HGVH7AFX2_n02_vk8-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVH7AFX2_n02_vk8-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6989882
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG201340.2880449197854842No Hit
CGGGAAGGAATAAGAAGCAACTAAACGAGGGTGTAGAAAAAGACGAAGAG145340.2079291181167293No Hit
CGGGAAGAGATTTAGTATGTGGGACAGAATGTCGGCGGCAGTATTGAGAC111380.15934460696189148No Hit
TGGGGTGGTATAGTCCGCATTGGGATGTTACTTTCCTGTTATGGCATGGA90580.12958730919921108No Hit
CGGCAAAATGTTAGTGCAGGAAAGCGGGAAGGAATAAGAAGCAACTAAAC84720.1212037628103021No Hit
TGGGTGGAGGACAATTTTTATTATATTTCATCTAATAGCAATAGGATATG79180.11327802100235741No Hit
GTGCAAGATGAATAGCCAGTGCAATATATACATGTATACTTAACAGATAT70440.10077423338476958No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGGC33350.026.76041430
CGGCGGC36250.024.52356133
CGGCAAG16750.024.0590611
GTGGGAC38150.023.30170419
TCGGCGG39150.022.974732
GTCGGCG38900.022.85244431
CCGTATC121100.022.45676255
CGTATCA121700.022.34604856
GTGCAGG33900.022.29988314
TCATTAA126650.022.02474860
CATTAAA129550.021.82889661
CGCGGAG5000.021.7263771
CGAGCAG15700.021.650491
CGGGAAG105900.021.5417331
CGGCAAA59250.021.46911
GTATCAT124600.021.32033557
CGCCGTA130700.021.20898653
CGCCAAG13250.021.1577631
GAATGTC42450.020.94164327
GCCGTAT137500.020.4655654
Produced by FastQC (version 0.11.5)