FastQCFastQC Report
Thu 27 Aug 2020
HGVH7AFX2_n02_vk8-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVH7AFX2_n02_vk8-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5113381
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG189190.36999003203555536No Hit
CGGGAAGAGATTTAGTATGTGGGACAGAATGTCGGCGGCAGTATTGAGAC84810.16585894929401898No Hit
CGGGAAGGAATAAGAAGCAACTAAACGAGGGTGTAGAAAAAGACGAAGAG80910.15823190174954693No Hit
TGGGGTGGTATAGTCCGCATTGGGATGTTACTTTCCTGTTATGGCATGGA75400.1474562525264595No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGGC27950.028.54994230
GTGGGAC29800.027.12988519
CGGCGGC32400.024.95279333
GAATGTC33700.023.8863727
CGCGAAG8900.023.2291131
AATGTCG35400.022.54155228
CGGGAAG69300.022.5007691
TCGGCGG35650.022.28530332
ATGTCGG37000.021.66137329
GGCGGCA38250.021.04498334
CGAGCAG11350.020.9934431
GTCGGCG38050.020.97164231
CGCCAAG10050.020.9197181
CCGTGTC14950.020.8369955
TATGTGG40850.020.81931516
CGGCAAG12500.020.743991
TAGTATG42200.020.48503513
GCGGCAG39450.020.40483335
GTCCGCA39850.020.28784213
GAAGCGT37550.019.85280469
Produced by FastQC (version 0.11.5)