FastQCFastQC Report
Thu 27 Aug 2020
HGVH7AFX2_n02_vk8-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVH7AFX2_n02_vk8-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9098352
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG276490.3038901990162614No Hit
CGGGAAGGAATAAGAAGCAACTAAACGAGGGTGTAGAAAAAGACGAAGAG234460.2576950199332802No Hit
CGGGAAGAGATTTAGTATGTGGGACAGAATGTCGGCGGCAGTATTGAGAC149670.16450231866166531No Hit
CGGCAAAATGTTAGTGCAGGAAAGCGGGAAGGAATAAGAAGCAACTAAAC127740.14039905248774723No Hit
TGGGGTGGTATAGTCCGCATTGGGATGTTACTTTCCTGTTATGGCATGGA113930.12522047948903275No Hit
TGGGTGGAGGACAATTTTTATTATATTTCATCTAATAGCAATAGGATATG93780.10307361157273318No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGGC44300.027.80932230
GTGGGAC50700.024.57499919
CGTATCA196800.024.38289856
CCGTATC197650.024.18949955
TCGGCGG51250.023.76494632
CGGCGGC50700.023.7468933
CATTAAA208300.023.72488261
TCATTAA206650.023.6433360
AAAGGGG97850.023.60731170
GTATCAT198100.023.51616757
GTGCAGG48300.023.40490314
GTCGGCG52850.023.3103731
CGCCGTA214400.023.16490753
GCCGTAT225450.022.30896854
ATCATTA218950.022.12402359
GAATGTC57500.022.03394327
TCGCCGT227800.021.69364252
AATGTCG58350.021.65298828
TATCATT220350.021.58635758
CGGCAAA90550.021.2069661
Produced by FastQC (version 0.11.5)