FastQCFastQC Report
Thu 27 Aug 2020
HGVH7AFX2_n02_vk8-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVH7AFX2_n02_vk8-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4930395
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG148580.30135516525552214No Hit
CGGGAAGAGATTTAGTATGTGGGACAGAATGTCGGCGGCAGTATTGAGAC89330.18118223793428315No Hit
TGGGGTGGTATAGTCCGCATTGGGATGTTACTTTCCTGTTATGGCATGGA82490.1673091101220085No Hit
CGGGAAGGAATAAGAAGCAACTAAACGAGGGTGTAGAAAAAGACGAAGAG79030.16029141681346018No Hit
CGGCAAAATGTTAGTGCAGGAAAGCGGGAAGGAATAAGAAGCAACTAAAC53990.10950441090419732No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGGC25300.031.81688330
CGGCGGC29100.027.90292433
GTGGGAC29750.027.41064519
GTGCAGG21700.026.2897814
TCGGCGG31150.026.17870732
GAATGTC31250.025.98320227
CGGGAAG69750.023.7603021
AATGTCG34400.023.50218428
GGCGGCA35100.023.23290334
GTCGGCG35450.022.70711131
CGAGCAG10300.022.1111411
GCGGCAG38150.021.8341935
CGGCAAG11150.021.6824971
CGGCAGT38550.021.42606236
GAAGCGT35750.021.04830469
CGCGAAA8000.021.0225941
CGGCCTG1506.328373E-721.0225931
GTCCGCA37350.020.89649213
TATGTGG40150.020.83372916
AGTCCGC37550.020.7851912
Produced by FastQC (version 0.11.5)