FastQCFastQC Report
Thu 27 Aug 2020
HGVH7AFX2_n02_vk8-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVH7AFX2_n02_vk8-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7405118
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG202450.27339199726459457No Hit
CGGGAAGGAATAAGAAGCAACTAAACGAGGGTGTAGAAAAAGACGAAGAG79170.10691254345980712No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA100150.025.75618753
CCGTATC100250.025.52102355
CGTATCA101050.025.2843456
GCCGTAT109000.024.17873454
TCGCCGT111500.024.0440352
GTCGCCG124200.023.44526151
GGTCGCC125750.023.40676150
GTATCAT108050.022.6421457
TGGTCGC126700.022.34730749
ATCTCGG122800.021.9741642
GATCTCG124700.021.58306941
CTCGGTG125750.021.48649444
CGCGCAG3800.021.2098451
AAAGGGG94050.021.09990770
TCTCGGT127100.021.01044743
CGCGGAG6050.020.8516461
TCATTAA132600.020.6668660
CATTAAA135050.020.57700761
AGATCTC133800.020.16747940
GTGGTCG141900.020.15096948
Produced by FastQC (version 0.11.5)