FastQCFastQC Report
Thu 27 Aug 2020
HGVH7AFX2_n02_vk8-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVH7AFX2_n02_vk8-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9484094
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG277500.2925951598539618No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGGC29700.025.09991530
CGGCCTA2650.023.8060531
CGGCGGC31450.023.36977633
CGGGCAG8100.022.9325121
CGAGCAG21150.022.2052271
TCGGCGG36000.020.90186332
CGGCAAG19950.020.2029951
CGCGAAG11000.020.0728341
TGCGGTG16450.019.3881451
CGCGAAA12450.018.8610651
GTGGGAC40100.018.85204319
CGTATCA127950.018.8200256
CGCGCAA5600.018.7756081
CCGTATC127550.018.7692855
CGGGAAG83100.018.72591
GAATGTC41400.018.344827
CGCCAAA25800.018.3389661
GTCGGCG40500.018.32018731
CGCCAAG15550.018.2564131
CGCCGTA140550.017.1577553
Produced by FastQC (version 0.11.5)