FastQCFastQC Report
Thu 27 Aug 2020
HGVH7AFX2_n02_vk8-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVH7AFX2_n02_vk8-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7121412
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG172120.24169364165421126No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGGC18150.021.59694730
CGCGGAG6150.021.0819851
CGGCGGC18850.020.98090233
CGAGCAG15550.019.1546151
CGCGAAG10000.018.9225061
CGGCAAG18000.018.6888961
CGCCAAG13900.018.4031851
CGGGAAG67850.018.3342631
TTAACCG36650.018.04916458
CGGGCAG7950.017.6310331
CGCGAAA10750.017.6023311
GTCGGCG22150.017.38080631
CGGGCTG3850.017.2932951
TAACCGT39700.016.75067559
CTAAACG39850.016.68692221
CGCGCAG2753.0377123E-916.5651571
TGCGGTG13550.016.551051
CGGGCAT6500.016.1730841
ACCGTAG42100.015.96159261
TCGGCGG24350.015.81046832
Produced by FastQC (version 0.11.5)