FastQCFastQC Report
Thu 27 Aug 2020
HGVH7AFX2_n02_vk8-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVH7AFX2_n02_vk8-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6526521
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG168780.2586063846266641No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA94300.026.09237753
CCGTATC93050.026.02913555
CGTATCA94600.025.49165756
TCGCCGT100700.024.43294752
GCCGTAT103750.024.1205954
GTCGCCG113850.023.91644151
GGTCGCC117350.023.41189850
AAAGGGG83200.022.75781870
TGGTCGC117100.022.71468549
ATCTCGG115700.021.84039742
GATCTCG116500.021.72012941
CTCGGTG118150.021.5356244
TCTCGGT117300.021.45297643
CGCGGAG5400.021.4162241
CGAGCAG17850.021.0072021
AGATCTC120050.020.93207740
CGGCCAG5150.020.4144041
GTATCAT115200.020.26484757
CGCGAAG8550.020.0841121
GTGGTCG134200.020.05536348
Produced by FastQC (version 0.11.5)