FastQCFastQC Report
Thu 27 Aug 2020
HGVH7AFX2_n02_vk8-16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVH7AFX2_n02_vk8-16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5196753
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG131040.25215745293262926No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACC3550.023.6669833
CGCGAAG6500.021.563151
CGAGCAG11700.020.3651981
CGGGCAG4350.020.1381
CGGCCAG3150.020.0229261
CGGCAAG12750.019.7873611
CGCGGAG3900.018.8677561
CGGCCTG2254.5747583E-918.6880651
TCGTGAC4900.018.5800082
CGGGCTG2701.2187229E-1018.1689531
TGCGGTG11400.018.13481
CGCGAAA7300.017.7600611
CGACAAG13050.016.915921
CGCCAAG8150.016.3377251
CGCCAAA16550.016.3026541
CACGGAG4000.015.7680541
CGGGAAG19050.015.63471
GCGGTAG18850.015.6040242
CGGCTAG1807.088901E-515.5733871
CGCACAG4100.015.3834671
Produced by FastQC (version 0.11.5)