FastQCFastQC Report
Thu 27 Aug 2020
HGVH7AFX2_n02_vk8-15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVH7AFX2_n02_vk8-15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4793782
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG93110.19423077645166176No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGCAG10900.025.3943041
CGGCAAG10750.024.4449161
CGGGCTA2350.022.3644961
CGCGAAA7100.020.2330441
CGCAAAG12900.019.5559311
CGGCCTG2353.7471182E-1019.3825631
TGCGGTG9450.019.2800081
CGCGAAG6050.018.5323431
CGCCAAG10100.017.692311
TGCGGTA9000.017.5188561
GGGCAGA12200.017.50882
CGCCAAT9150.016.8487341
GCGGTAG22100.016.7957732
CACCTCG7000.016.49951634
GCGAAGA21450.016.4884742
AGCGGTA19050.016.3693241
CCGGGAG2201.3814915E-615.9262321
TATACCG15200.015.65743417
TGGGCAG9850.015.6513631
CGCCATG3855.456968E-1215.4711961
Produced by FastQC (version 0.11.5)