FastQCFastQC Report
Thu 27 Aug 2020
HGVH7AFX2_n02_vk8-14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVH7AFX2_n02_vk8-14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5924694
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG161840.27316178692097853No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAG8150.024.5125471
CGAGCAG14700.021.6967771
CGGCAAG16000.021.248241
CGCGAAA9250.018.9452111
CGGGAAG26050.018.028861
TGCGGTG11550.017.2967321
CGGCTAG3053.8198777E-1117.2370381
CGCGCAG2954.292815E-1016.6332531
CGCCAAG10650.016.4547621
CGGCCAG3900.016.1762961
CGCGGAG5350.015.7227551
GCGGTAG24250.015.5965062
CGGCAAT15800.015.527881
CGCGGAT4550.015.4059971
GTACGCG4350.015.28689860
CGACAAG17250.015.0353591
CGCGGAA5450.014.7911711
CGCCAAT12100.014.7725681
CGTGTCG4051.2732926E-1114.69091770
TACGGAG8250.014.444291
Produced by FastQC (version 0.11.5)