FastQCFastQC Report
Thu 27 Aug 2020
HGVH7AFX2_n02_vk8-13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVH7AFX2_n02_vk8-13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4595946
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG101190.22017229967453925No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAG3000.021.0234361
CGAGCAG10500.021.0234361
CGCGAAG5500.020.3863621
CGACCAG3300.020.1740041
CGGCAAG11950.019.3521161
CGGGCAG5150.018.3699911
CGCGAAA7400.017.519531
TGCGGTG7600.017.0584891
CGACAAG11200.016.8938311
CGCCAAA15950.016.2563651
CGCCGTG6300.016.11066670
CGCCAAT9550.015.7767491
CGGCAAC8550.015.5729151
CGGCAAT11100.015.4676931
GCGGAGC4450.014.94340726
CGGGCAA4251.8189894E-1214.8400711
CGGGAAG18500.014.5838241
GGGCAGA12550.014.2292172
TCGGGGC1750.001018974913.99961335
TGCGGTA9300.013.940271
Produced by FastQC (version 0.11.5)