FastQCFastQC Report
Thu 27 Aug 2020
HGVH7AFX2_n02_vk8-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVH7AFX2_n02_vk8-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6731382
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG213440.3170819899984877No Hit
CGGGAAGGAATAAGAAGCAACTAAACGAGGGTGTAGAAAAAGACGAAGAG167730.24917617214414517No Hit
TGGGGTGGTATAGTCCGCATTGGGATGTTACTTTCCTGTTATGGCATGGA153220.22762042029407925No Hit
CGGGAAGAGATTTAGTATGTGGGACAGAATGTCGGCGGCAGTATTGAGAC148870.22115815147617535No Hit
TGGGTGGAGGACAATTTTTATTATATTTCATCTAATAGCAATAGGATATG93400.13875308220511032No Hit
CGGCAAAATGTTAGTGCAGGAAAGCGGGAAGGAATAAGAAGCAACTAAAC86460.12844316367723596No Hit
GTGCAAGATGAATAGCCAGTGCAATATATACATGTATACTTAACAGATAT85310.12673474778284757No Hit
GCGGCAGTATTGAGACCATGAGAGTAGCAAACGTAAGTCTAAAGGTTGTT79020.11739045563006228No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGGC46400.031.3028230
GTGGGAC50750.028.89557519
CGGCGGC52100.027.87813633
TCGGCGG53100.027.35312532
GAATGTC53200.026.9727727
AATGTCG55350.026.24120728
GTCGGCG55650.026.09974731
GAAGCGT57250.023.903369
AAGCGTA58450.023.77182870
ATGTCGG62000.023.48307629
GGCGGCA62600.023.14618334
CGGGAAG129450.022.8739221
CGGGCAG6900.022.8533231
TAGTATG66250.022.55772613
CGAGCAG12050.022.391831
TTAACCG74650.022.2703458
CAGAATG65350.022.2257225
TATGTGG67500.022.0881416
TGAAGCG62150.022.0750468
CGCGAAG7050.021.8700351
Produced by FastQC (version 0.11.5)