FastQCFastQC Report
Thu 27 Aug 2020
HGVH7AFX2_n02_vk8-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVH7AFX2_n02_vk8-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6142086
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG209990.34188710480445894No Hit
CGGGAAGAGATTTAGTATGTGGGACAGAATGTCGGCGGCAGTATTGAGAC145280.23653201860084666No Hit
TGGGGTGGTATAGTCCGCATTGGGATGTTACTTTCCTGTTATGGCATGGA140550.22883105186088243No Hit
CGGGAAGGAATAAGAAGCAACTAAACGAGGGTGTAGAAAAAGACGAAGAG119420.19442905879207814No Hit
GCGGCAGTATTGAGACCATGAGAGTAGCAAACGTAAGTCTAAAGGTTGTT74140.1207081763426953No Hit
TGGGTGGAGGACAATTTTTATTATATTTCATCTAATAGCAATAGGATATG74070.12059420854738927No Hit
GTGCAAGATGAATAGCCAGTGCAATATATACATGTATACTTAACAGATAT70240.1143585420327882No Hit
CGGCAAAATGTTAGTGCAGGAAAGCGGGAAGGAATAAGAAGCAACTAAAC65370.1064296397022119No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGGC43300.029.0980430
GTGGGAC45050.028.0456219
CGGCGGC47000.026.73309333
TCGGCGG47800.026.13902732
CGTATCA126750.025.84649856
CCGTATC127900.025.61410555
CATTAAA133550.025.44690961
GAATGTC50100.025.21846227
GAAGCGT53000.025.02802569
AAAGGGG70700.024.99972370
CGAGATC15550.024.7579655
GTATCAT130200.024.59710557
CGCCGTA136500.024.41058353
CGCGCAG2450.024.3139481
TCATTAA137000.024.21851360
AAGCGTA55250.024.07213270
GTCGGCG52350.023.80029931
AATGTCG53350.023.74779128
GCCGTAT145000.023.29341554
GTGCAGG27750.023.08045214