FastQCFastQC Report
Thu 27 Aug 2020
HGVH7AFX2_n02_vk8-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVH7AFX2_n02_vk8-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5591940
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG179730.32140902799386256No Hit
CGGGAAGAGATTTAGTATGTGGGACAGAATGTCGGCGGCAGTATTGAGAC94870.16965489615410753No Hit
TGGGGTGGTATAGTCCGCATTGGGATGTTACTTTCCTGTTATGGCATGGA88540.15833503220706946No Hit
CGGGAAGGAATAAGAAGCAACTAAACGAGGGTGTAGAAAAAGACGAAGAG76980.13766242127061448No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGGC30000.027.76537730
CGGCGGC31350.026.23576433
TCGGCGG32700.025.36579332
GTGGGAC33100.025.05925819
CGAGCAG11350.024.383431
GAATGTC35450.023.39827527
CGGCAAG11550.023.3545911
GTCGGCG36150.022.9449931
CGGGCAG5100.022.6676921
GAAGCGT34950.022.63208869
AATGTCG38050.021.79944428
CATTAAA114600.021.25612661
GGCGGCA38950.021.02673134
CGTATCA112750.020.98500456
AAGCGTA37700.020.98120770
TCATTAA115000.020.93871960
TGCGGTG10400.020.8843961
CCGTATC114050.020.77649555
GTATCAT112100.020.60711757
GTCCGCA38650.020.55550213
Produced by FastQC (version 0.11.5)