FastQCFastQC Report
Thu 27 Aug 2020
HGVH7AFX2_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVH7AFX2_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10623242
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6599336.21216197465896No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC29100.021.78670755
TGTCGGC8850.020.95600730
CGCCGTA32150.020.37354553
CGTATCA31850.020.24029556
GCCGTAT35400.019.5915154
CCGGATC4350.019.32537855
CTAGATC5700.019.0075785
TCGCCGT35500.018.63643852
TCATTAA38150.018.08951260
CGGGAAG25650.017.3222481
TAGTCCG10550.017.2253611
GTATCAT35100.017.16831657
AGTCCGC11100.016.68669512
GTGGGAC12800.016.6807919
ACCGTAG13450.016.40867261
AAGATCG30150.016.3445056
CGAGATC7100.016.2440975
CTAAACG14300.016.15506621
GCAACTA15000.015.86778817
GATCTCG40650.015.84382841
Produced by FastQC (version 0.11.5)