FastQCFastQC Report
Thu 27 Aug 2020
HGVH7AFX2_n01_vk8-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVH7AFX2_n01_vk8-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6623422
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTTG201150.035.060666
CGGGCTG4900.034.99970226
GCTTGAA194650.034.6850468
TGCTTGA191350.034.2040567
CCGGGCT5300.031.69784225
TCTGCTT230200.030.83379665
AGGCCGC6150.030.73144516
GCCGTCT263500.030.29765558
CCGTCTT267150.030.22433559
GTCTTCT261400.029.17534661
CTTGAAA239150.028.6261469
TGCCGTC290400.028.37097257
TTGAAAA244200.028.30647770
CTTCTGC272950.027.8253763
CGTCTTC293550.027.42269160
ATGCCGT298300.027.37321756
ACACTCT44500.027.2222941
TTCTGCT259950.027.18384464
TGAAAAA152550.027.1416970
TATGCCG312850.026.4581455
Produced by FastQC (version 0.11.5)