FastQCFastQC Report
Thu 27 Aug 2020
HGVH7AFX2_n01_vk8-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVH7AFX2_n01_vk8-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6989882
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCTG4950.028.98953426
GGGTGCA10300.026.8443266
GCGTGCA13600.026.24966866
CGCCGGG5500.026.09057823
CCGGGCT5900.024.32172625
AGGCCGC6600.023.33303616
GCCCACA22100.022.815261
GAGATCG52950.021.1535786
GCCGTCT50650.021.14484656
GGGCTGC7550.019.93352327
TGCGTGC13600.019.30122665
CTGCGTG18650.019.14184864
TGCCGTC68200.018.9880355
CCGCCGG7750.018.96750322
GCCGGGC7400.018.9186824
GTGCAAA35050.018.5732368
TATGCCG69750.018.56607453
GAGGCCG8300.018.5539815
CTGCTTG46100.018.4487964
CCGCGTG3850.018.1815964
Produced by FastQC (version 0.11.5)