FastQCFastQC Report
Thu 27 Aug 2020
HGVH7AFX2_n01_vk8-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVH7AFX2_n01_vk8-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5113381
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCTG3000.026.83318326
ACACTCT33650.026.0104581
CCGGGCT3650.023.97246725
CTGCTTG65700.023.7593864
GCCCACA24550.023.67281
GCGTGCA6100.023.52445866
GCTTGAA61400.023.02918666
TGCTTGA61900.022.27774265
AGGCCGC4000.020.99988416
CGCCGGG4200.019.99988723
GGGCTGC4350.019.31023627
CCGTCTT101150.018.9618357
GCCGTCT99750.018.80691356
TCTGCTT90250.017.95557863
GGCCGCC4600.016.73903717
GCCACAC27300.016.414981
TGCCGTC124200.016.25997455
CTGCGTG11200.016.24990864
GTCTTCT108500.016.06442659
GTTGGGA15050.016.04642129
Produced by FastQC (version 0.11.5)