FastQCFastQC Report
Thu 27 Aug 2020
HGVH7AFX2_n01_vk8-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVH7AFX2_n01_vk8-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9098352
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCTTCTGAAGAGTTAAGCACTCCATTATGCTCATTGGGTTGCTACTA98430.10818442724572538No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGCT6750.034.22190525
CGGGCTG6850.031.6785426
AGGCCGC8200.029.45094716
CTGCTTG141300.027.79167764
GCGTGCA10600.027.73559466
GCTTGAA130150.027.21450466
CGCCGGG8250.027.15126823
TGCTTGA131600.026.11678165
GCCGTCT176450.025.40923156
GCCCACA36350.024.56111
CCGTCTT187400.024.42880457
TGCCGTC208800.023.34988255
GGGCTGC9700.023.09257127
ATGCCGT208900.022.78581454
TCTGCTT180050.022.2769463
TATGCCG220400.022.0415453
GTCTTCT203150.021.05321559
GTATGCC236200.020.89311852
GAGGCCG11100.020.8106215
GCCGGGC11500.020.69546324
Produced by FastQC (version 0.11.5)