FastQCFastQC Report
Thu 27 Aug 2020
HGVH7AFX2_n01_vk8-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVH7AFX2_n01_vk8-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4930395
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACA23150.035.089791
GCCACAC25550.025.0786381
GCGTGCA7800.022.88435666
CCGGGCT3200.020.78101525
CGGGCTG3250.020.46130626
ACACTCT25850.020.1822431
TTGCGGG9600.019.68727955
GCGGGGT10400.019.18247457
TGCGGGG7950.017.16961956
GTCGGGG2451.3315002E-817.14266462
GGGTGCA7150.017.13267366
CGGGGTG11000.016.86344558
GCACCAC22050.016.5146181
TGCGTGC7350.015.71410865
TGGCGGG7000.015.49982555
GAGATCG35550.015.3595666
GTGCAAA21450.015.01148468
TTTGCGG14100.014.39754
GCACCCA16950.014.2535531
GCATGGG13550.014.20648145
Produced by FastQC (version 0.11.5)