FastQCFastQC Report
Thu 27 Aug 2020
HGVH7AFX2_n01_vk8-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVH7AFX2_n01_vk8-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7405118
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGGT26850.026.98299651
ATCGGGG75800.025.39554849
TCGGGGG34950.025.23582350
AATCGGG81650.024.64766348
GAGATCG47650.023.2121126
GAATCGG89350.022.95446447
GCGGGGT25600.021.73808559
TTGCGGG23500.021.59554957
TGGCGGG17300.021.04027257
GCCCACA20950.020.3884221
GGAATCG132800.019.81909646
TGCGGGG21400.019.7895458
CGGGGTT41650.019.57965351
CGGAATC162200.019.0750445
TCGGGGT46300.018.74713150
CGCGGTT12450.018.55404951
TGCGCGA5100.018.52924312
GTCGGGG5150.018.34934864
TCGCGGT14150.018.05637450
TACGCGC3750.017.73317122
Produced by FastQC (version 0.11.5)