FastQCFastQC Report
Thu 27 Aug 2020
HGVH7AFX2_n01_vk8-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVH7AFX2_n01_vk8-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9484094
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACA36700.027.8587471
GCGTGCA11950.027.23823468
CGAGGGC5300.024.433657
CCGGGCT5700.022.10493525
GCCGTCT69600.021.72381658
CGGGCTG5900.020.76240326
CTGCTTG57850.020.50974366
TGCCGTC85750.020.16296657
GGGATTC24850.019.4363332
GGCGGGT6150.019.357471
CTGCGTG16550.019.0330566
TGCGTGC11500.018.86938567
ACCGAGC7400.018.44576851
TATGCCG93700.018.3029255
ATGCCGT87500.018.19982756
CCGTCTT89700.018.06550659
GCCACAC34100.017.9691891
GTACGCG5100.017.84296621
CCGGTTG9000.017.11094918
TACGCGC5350.017.00918422
Produced by FastQC (version 0.11.5)