FastQCFastQC Report
Thu 27 Aug 2020
HGVH7AFX2_n01_vk8-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVH7AFX2_n01_vk8-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7121412
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACA23500.025.7745951
CCGGGCT3255.456968E-1217.23061625
GCCGTCT52400.016.2307758
CTGCTTG50400.015.76374866
GCTTGAA49650.015.43792968
TGCGCGA4850.014.43286112
TGCTTGA53150.014.15791767
TGCCGTC62650.013.85462557
GTGCGCG5350.013.73819511
CCGTCTT62500.013.71987859
AGTGCGC5400.013.6109910
CGGGCTG4203.2923708E-1013.33321426
CGCCGGG4253.9835868E-1013.176352523
TATGCCG66600.012.82270855
ATGCCGT67800.012.59575856
GCGCGAT5651.8189894E-1212.3892713
GAGTGCG6000.012.2498919
CGCGATC5206.002665E-1112.11527614
ACACTCT28250.011.6499211
GTATGCC76000.011.37489954
Produced by FastQC (version 0.11.5)