FastQCFastQC Report
Thu 27 Aug 2020
HGVH7AFX2_n01_vk8-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVH7AFX2_n01_vk8-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6526521
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACA19350.028.9507981
GCCGTCT85500.028.36822958
CTGCTTG72300.028.36771866
TGCCGTC100650.026.77577657
TATGCCG104300.025.83874955
CCGTCTT98100.025.29544659
ATGCCGT106250.025.16688756
TGCTTGA73700.024.17215767
GTATGCC113400.024.01218454
GCTTGAA77300.023.7708668
CTCGTAT117500.023.26367251
GAGATCG42050.023.222736
CGTATGC118500.022.8901453
CATCTCG121250.022.80396848
TCGTATG119050.022.63739452
TCTCGTA120550.022.50088350
ATCTCGT123100.022.37596349
TCTGCTT95500.022.17262565
GTCTTCT108650.021.42185261
CGTCTTC120100.020.77837660
Produced by FastQC (version 0.11.5)