FastQCFastQC Report
Thu 27 Aug 2020
HGVH7AFX2_n01_vk8-16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVH7AFX2_n01_vk8-16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5196753
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACA35150.026.8930661
ACACTCT22500.024.5853541
GGTCACG3900.022.43568812
GCCACAC30150.020.9019621
CACTCTT39350.018.0614741
GCACCCA18900.014.8193821
AGTGGCG6650.014.73670539
GCGCGCC2151.978268E-514.65102569
GTGCGCG2202.4576222E-514.318048511
AGGGGGG2759.145897E-714.0043151
AGCGCAT6750.013.48135560
GGCCGCG1900.001894075112.89461653
GTCACGA6850.012.77360313
GTGGCGG7700.012.72715440
ATGCCGG9350.012.72715470
AGTGCGC2508.1663806E-512.59988310
CGGTTGC7050.012.41123119
GAGTGCG3407.3356387E-712.3528269
AGGTCAC6250.012.31988511
CCGGTTG6850.012.2626618
Produced by FastQC (version 0.11.5)