FastQCFastQC Report
Thu 27 Aug 2020
HGVH7AFX2_n01_vk8-15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVH7AFX2_n01_vk8-15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4793782
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT34400.040.3042531
CGCTTGC8050.029.99976223
GCGCTTG8550.028.65474322
GCCCACA31850.026.9321371
CACTCTT57550.026.0991331
GCGGTAG7650.024.70568743
AGTGGCG8000.024.49980739
TAGAGCC11700.022.1375035
CCGAGTG3900.021.53829261
ACAGGCT9200.021.3050694
TGGCGAT8050.020.869453
AACAGGC9450.020.7414443
CGAGTGG4050.020.74057662
GGCGGTA9400.020.47856142
AGGCTTG9750.020.4622296
GGCGATT8800.020.2839354
AGTCCAG11750.020.25515716
TTAGAGC13450.020.0378534
GAGCTGC9800.019.99984232
GTGGCGG10250.019.46326340
Produced by FastQC (version 0.11.5)