FastQCFastQC Report
Thu 27 Aug 2020
HGVH7AFX2_n01_vk8-14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVH7AFX2_n01_vk8-14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5924694
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT35150.032.273681
GCCCACA25800.027.6846661
AGTGGCG8750.022.39978439
CACTCTT61950.019.4987431
GCCACAC25400.019.2984831
GCGGTAG10200.019.21550243
GGCGGTA10650.018.73221442
GTGGCGG10600.018.16020440
GAGCTGC12500.016.23984332
TGGCGGT12100.016.1981941
GGCGATT11500.015.82593354
CGGTAGT12650.015.77059944
ACAGGCT13100.015.2293744
TGGCGAT12600.014.99985553
TGAGCTG13300.014.99985531
AGGCTTG13850.014.9101066
GTTGCCG13000.014.53844469
AACAGGC13450.014.31261353
GCGTGCA4650.014.30105970
TTGGCGA14200.014.29563752
Produced by FastQC (version 0.11.5)