FastQCFastQC Report
Thu 27 Aug 2020
HGVH7AFX2_n01_vk8-13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVH7AFX2_n01_vk8-13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4595946
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT24650.032.3828471
GCCCACA26850.026.6000821
AGTGGCG6700.025.07443839
GTGGCGG7100.024.1547540
CGCTTGC7200.022.84705223
GCGGTAG7450.021.61057943
CACTCTT41300.021.1927381
GTTGCCG7300.021.09573469
GGCGGTA8050.020.43463142
GGTTGCC7450.020.20119368
GCGCTTG8300.018.97576122
AGGCTTG9450.018.8895686
GCACCCA14850.018.8608251
CGGACAG3450.018.26073348
CGGTAGT9350.017.96778144
TAGAGCC9600.017.8652275
TGGCGGT9600.017.49986841
GCCACAC27250.017.4730821
TCGCGGA4050.017.28382345
TTCGCGG3650.017.26014544
Produced by FastQC (version 0.11.5)