FastQCFastQC Report
Thu 27 Aug 2020
HGVH7AFX2_n01_vk8-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVH7AFX2_n01_vk8-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6731382
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCTG4250.037.88226
GCCCACA28350.031.740361
CCGGGCT5100.031.5683325
CGCCGGG5550.029.63936223
GCTTGAA68550.029.10257368
CTGCTTG73600.028.57989566
TGCTTGA69450.027.71752467
ACACTCT45250.027.2367861
GGGCTGC5950.027.0585727
AGGCCGC6300.026.66641816
GAGGCCG7350.023.80930115
GGCCGCC7250.022.68944417
GCCGGGC7350.022.38074324
TCGAGGC8150.021.04274713
CCGCCGG7850.020.50936322
CCGTCTT112050.020.24077259
GCCGTCT112400.020.20888558
TCTGCTT107550.019.6557965
GCCACAC29200.018.9454961
CCTACGA7400.018.91874136
Produced by FastQC (version 0.11.5)