FastQCFastQC Report
Thu 27 Aug 2020
HGVH7AFX2_n01_vk8-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVH7AFX2_n01_vk8-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6142086
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGGG17000.029.44086657
GTCGGGG5300.028.3959364
GCGGGGT26500.027.86763259
GGGTGCA9300.027.09648968
GCGTGCA5850.026.92279668
AGGCCGC5000.026.59972216
CGGGCTG5150.026.50457626
CGGGGGT21950.026.46918951
CGGGGTC11800.026.39802760
ATCGGGG65900.026.1304449
GCCCACA24300.025.3579941
TTGCGGG24100.025.26944457
AATCGGG73450.024.92165848
TCGGGGG27750.024.59453850
GCGGCGT11100.024.5943459
CCGGGCT5600.024.37474625
TGCGGGG20800.024.23051658
CGGGGTT35450.024.09021251
GGGTCGG5150.023.78615862
GAATCGG77750.023.6782147
Produced by FastQC (version 0.11.5)