FastQCFastQC Report
Thu 27 Aug 2020
HGVH7AFX2_n01_vk8-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVH7AFX2_n01_vk8-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5591940
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGCA11550.034.84820668
AGGCCGC4600.030.43453616
GCCCACA24550.029.804621
TGCGTGC9900.028.282667
CGGGCTG4350.027.35610226
CCGGGCT4700.025.31894525
CTGCGTG13700.024.01440666
GTCGGGG3100.023.70948664
TGGCGGG5350.023.55121457
CCGCGTG2750.022.90890566
GCCGTCT38700.022.88095358
GAGGCCG5750.022.52155915
ACACTCT31450.022.4863821
CGCCGGG5300.022.4526523
GTGCAAA26600.021.97350970
GGGTGCA8150.021.4722268
GGGATTC15850.021.41938632
CGTGCAA19250.021.09073869
GTCCGCG2402.3646862E-1120.41650264
TGCCGTC52500.020.3331757
Produced by FastQC (version 0.11.5)