FastQCFastQC Report
Thu 27 Aug 2020
HGVH7AFX2_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVH7AFX2_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10623242
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2582742.431216383849676No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACA20200.029.427751
GCCACAC19950.019.9811941
GAGATCG31050.017.5637056
TGAGATC13050.015.5371265
GCACCCA11350.015.0959241
CCGAGAC3755.4569682E-1114.91788829
GGAGATC7450.014.5465035
GAAGATC18300.014.5182975
GAGACCG4051.8735591E-1013.81318531
CACTCTT45550.013.4341191
ACGTCCG6950.013.10849239
AGACCGA4558.54925E-1113.06450532
TCACAGT26600.012.51428937
CGAGGGC2852.005943E-512.29752457
CGAGACC4551.1878001E-912.29519430
CGCGGAG3204.950529E-612.04795868
GCGTGCA3551.2162691E-611.84691267
AAGATCG72600.011.8454536
CGTCTGA145450.011.74327123
TGAACTC142500.011.67902627
Produced by FastQC (version 0.11.5)