FastQCFastQC Report
Fri 28 Aug 2020
HGVGWAFX2_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVGWAFX2_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41956108
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG311400.015.318556145
CACACGT354900.012.635746145
ACACGTC341750.012.54304145
CGTCTGA305450.012.52235145
CACGTCT322700.012.493719140-144
GCACACG415800.012.423962145
AGCACAC477200.012.345569145
AGAGCAC546550.011.704148145
TAGATCG325400.011.416839145
AGATCGG897700.010.774526145
TCGGAAG737800.010.26089140-144
GATCGGA797400.010.134252140-144
TCTGAAC456050.010.122401145
GAACTCC328300.010.110736145
CGGAAGA758550.09.875761140-144
ATCGGAA902250.09.4532585145
ACTCCAG345600.09.032154145
GAAGAGC865600.08.837688145
GGAAGAG912350.08.698061145
CACACGG112050.08.632121145
Produced by FastQC (version 0.11.5)