FastQCFastQC Report
Fri 28 Aug 2020
HGVGWAFX2_n01_S4new.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVGWAFX2_n01_S4new.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25410348
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGAGCC187300.019.77946145
GAGCACC244900.014.032042145
CACACGG127100.013.518674145
CGGAAGC245050.010.916992145
AACTCCC116200.09.857851145
CAGTCAC497150.08.9539145
AGTCACA473900.08.934237145
GAACTCC758900.08.473648145
ACGTCTG1003650.08.40818145
TGAACTC852900.08.21127145
TCTGAAC927050.08.203585145
GTCACAT454550.07.958848145
TCCAGTC618500.07.947331145
AGCACAC1394200.07.852052145
ACACGTC1126350.07.839805145
TCACATC487300.07.810776145
CCAGTCA533600.07.7448397140-144
ACTCCAG659600.07.7379036145
GTCTGAA1033000.07.6499305145
CGTCTGA948450.07.598455140-144
Produced by FastQC (version 0.11.5)