FastQCFastQC Report
Fri 28 Aug 2020
HGVGWAFX2_n01_S3new.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVGWAFX2_n01_S3new.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30395540
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGAGCC200500.025.70911145
AACTCCC110450.013.127926145
GAGCACC378000.012.466753145
CACACGG184800.012.279317145
CGGAAGC420100.010.527105145
AGTCACC528450.08.492167145
CAGTCAC564500.07.8470984145
GAACTCC885000.07.7332234145
AGCACAC1466500.07.5489893145
TCTGAAC906700.07.524157145
TCCAGTC752450.07.486438145
GTCTGAA956000.07.4243193145
ACACGTC1174500.07.3887844145
TGAACTC931850.07.3755455145
ACGTCTG1036400.07.2890706145
CGTCTGA944500.07.25718140-144
ACTCCAG798800.07.0520406145
AGAGCAC1588650.07.0324435145
CCAGTCA581600.06.926153140-144
CTCCAGT709400.06.816579145
Produced by FastQC (version 0.11.5)