FastQCFastQC Report
Fri 28 Aug 2020
HGVGWAFX2_n01_S2new.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVGWAFX2_n01_S2new.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29633956
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGAGCC233350.018.98302145
GAGCACC236550.017.071203145
CACACGG122950.015.272254145
CGGAAGC195200.014.782067145
AGTCACC146150.011.210927145
ACAGTGC213150.09.591702145
GTCACAC831250.09.393252145
TCCAGTC1168000.08.621668145
CACAGTG731250.08.615606145
CCAGTCC187950.08.601901145
CAGTCAC977400.08.522747145
ACACAGT756650.08.21141145
CACACAG844100.08.0563965145
AACTCCC176600.08.04632145
CTCCAGT1228050.07.881287145
AGTGATG538400.07.7966037145
GAACTCC1460000.07.6769476145
ACTCCAG1367400.07.475762145
CCAGTCA1035950.07.4185987140-144
AGTCACA961600.07.411632140-144
Produced by FastQC (version 0.11.5)