FastQCFastQC Report
Fri 28 Aug 2020
HGVGWAFX2_n01_I4new.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVGWAFX2_n01_I4new.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16022150
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACC106700.012.162381145
AAGAGCC103950.010.670798145
CACACGG57850.010.151066145
CACTTAG187100.010.113389145
AGGCATG98000.09.617169145
GAACTCC439300.09.357327145
CAGTCAC284950.09.23566145
CCAGTCA310200.08.951317145
ACTTAGG177850.08.682718145
TCCAGTC339900.08.446445145
ACGTCTG585900.08.290511145
GTCACTT299400.08.208766145
CGTCTGA569750.08.131047145
CACGTCT622350.07.991336145
ACACGTC655150.07.9453616145
ACTCCAG369450.07.908231145
AGCACAC810350.07.747743145
CGGAAGC83400.07.736667145
AGTCACT310800.07.7210603145
TCACTTA282900.07.7137136145
Produced by FastQC (version 0.11.5)