FastQCFastQC Report
Fri 28 Aug 2020
HGVGWAFX2_n01_I3new.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVGWAFX2_n01_I3new.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20716374
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGAGCC146750.016.59939145
GTAATGG118900.010.548608145
CCAGTCC105000.010.356973145
CACACGG105200.09.648131145
GAGCACC259050.09.151559145
CGTATGG56400.08.740992145
CGGAAGC169000.08.62264145
AGTCACC497000.08.621087145
ACCTTGT415700.08.510806145
GTAATCG93050.08.336779145
CACCTTG446800.08.242927145
CAGTCAC546400.08.199903145
ACTCCAG703950.07.878627145
TGTAATG259100.07.834685145
TCCAGTC646150.07.7979846145
CTTGTAA421600.07.56629145
CCTTGTA374950.07.502211145
CGTCTGA1032450.07.41525145
TCACCTT528500.07.297894145
GTCACCT492900.07.266061145
Produced by FastQC (version 0.11.5)