FastQCFastQC Report
Fri 28 Aug 2020
HGVGWAFX2_n01_I2new.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVGWAFX2_n01_I2new.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28726431
Sequences flagged as poor quality0
Sequence length151
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGTCC66700.010.434625145
GAGCACC150750.010.051257145
CGGAAGC125550.09.354697145
GTCACGC388500.09.106684145
AAGAGCC185250.08.962078145
CACGCCA367200.08.746463145
TCACGCC380650.08.551689145
ACGTCTG956000.08.273671145
CAGTCAC519700.07.9934335145
ATGCCGG79450.07.847584145
CGTCTGA935850.07.715863145
GAACTCC732550.07.6601114145
ACGCCAA406850.07.573315145
ACACGTC1066500.07.4300327145
CACACGG89950.07.415118145
AGTCACG440250.07.377515145
GCACACG1169850.07.300398145
CACGTCT1019000.07.14317145
AGAGCAC1510250.07.123878145
CACACGT1124000.07.095088145
Produced by FastQC (version 0.11.5)