FastQCFastQC Report
Mon 3 Apr 2017
HGV7YBGX2_n01_9w-59_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGV7YBGX2_n01_9w-59_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16968795
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG200880.11838200650075624No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG34100.038.3413315
TCGTATG30150.037.64402445
CTCGTAT26900.037.44719344
TATGCCG32200.035.89034748
CACGTCT38850.033.5651214
ACACGTC41300.031.82483913
TGTTACC35600.030.91234413
CGTATGC40800.028.9171846
AGTCACA42250.028.82113628
CAGTCAC43000.028.39866427
GTATGCC43950.027.00160447
TCTAGTT49250.026.196077
CTAATGC41950.025.82004421
ATGCCGT44550.025.7861549
GTCTGAA52350.025.50123617
GTCACAT47950.025.32311829
CGCTATG47900.025.13350329
CTATAAG67050.024.5419846
TTACCTC44700.024.54154615
TCTAATG44200.024.34958620
Produced by FastQC (version 0.11.2)