FastQCFastQC Report
Mon 3 Apr 2017
HGV7YBGX2_n01_9w-53_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGV7YBGX2_n01_9w-53_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85522017
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG1344260.15718291583324093No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG1071240.12525897278592013No Hit
CTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACGGCGCCGGAGGCACGACCCGGCCAGTTAAGGCCAGGAGCGC986340.11533170458316014No Hit
CTTTGAGCACTCTAATTTCTTCAAAGTAACGGCGCCGGAGGCACGACCCGGCCAGTTAAGGCCAGGAGCGCATCG965530.11289841304842002No Hit
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGCCATCCCTCCGC894920.10464205959969348No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG166600.043.4826943
CTCGTAT161300.042.64388742
AGTCACG177550.042.02396428
ACGTCTG198600.040.7854415
TATGCCG183050.040.0476346
CACGGCT204100.036.26983631
CACGTCT226300.035.85473314
ACACGTC246500.033.2526113
CAGTCAC235850.032.45507427
CGTATGC228850.031.8366644
GTATGCC233550.031.37339445
ATGCCGT241500.030.35497747
TGTTACC181750.028.88926313
GCTACAT252300.028.84864235
GTCACGG275300.027.95468129
GTCTGAA295150.027.5942917
TTACCTC198500.026.50303815
CTATAAG421550.025.92529346
CTAATGC203400.025.67632121
TCTAGTT239050.025.2820137
Produced by FastQC (version 0.11.2)