FastQCFastQC Report
Mon 3 Apr 2017
HGV7YBGX2_n01_9w-51_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGV7YBGX2_n01_9w-51_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36587599
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC498370.1362128189936705No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA383310.10476500521392508TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG73300.045.2740845
CTCGTAT64450.045.0143544
ACGTCTG87000.043.02178215
TATGCCG77800.042.5671848
CACGTCT96350.039.24123414
CAGTCAC96200.036.25195327
CACAGTC95200.035.7991231
ACACGTC108300.035.7715813
AGTCACA96950.035.75803428
TGTTACC108350.035.11829413
GTCACAG99700.034.32194529
GTATGCC100650.033.24618547
CGTATGC101200.033.0994546
TTACCTC116000.032.623215
ATGCCGT104050.032.22603249
TCTAGTT132000.031.4376037
GTCTGAA121050.031.23226517
ACAGTCA110850.030.86949532
CTAATGC123900.030.59686921
TTTTTCC195000.030.3107191
Produced by FastQC (version 0.11.2)