FastQCFastQC Report
Mon 3 Apr 2017
HGV7YBGX2_n01_9w-50_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGV7YBGX2_n01_9w-50_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15959780
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC243640.15265874592256284No Hit
CTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACGGCGCCGGAGGCACGACCCGGCCAGTTAAGGCCAGGAGCGC192340.12051544570163247No Hit
CTTTGAGCACTCTAATTTCTTCAAAGTAACGGCGCCGGAGGCACGACCCGGCCAGTTAAGGCCAGGAGCGCATCG192120.12037759918996377No Hit
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG183800.11516449474867448No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATC159770.10010789622413342No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG35050.042.8132915
CTCGTAT26500.042.3063342
TCGTATG30650.041.9807643
TATGCCG32900.039.5307546
TGTTACC51500.036.77482613
ACACGTC42750.035.34463513
TTACCTC54750.034.84322715
CACGTCT43850.034.45789314
TAGTTGT56050.034.2202649
CAGTCAC41250.032.69724327
TTTTTCC99050.032.292421
GTATGCC40800.032.04551745
CTAATGC58650.031.5833221
TACCTCT61100.031.22076816
TCTAGTT67200.030.9533547
TCTAATG61850.030.17244120
CGTATGC42850.030.02927844
GTCTGAA50550.029.75232717
TTTTCCA111700.029.308472
ATGCCGT45150.028.80535347
Produced by FastQC (version 0.11.2)