FastQCFastQC Report
Mon 3 Apr 2017
HGV7YBGX2_n01_9w-49_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGV7YBGX2_n01_9w-49_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21681552
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG332280.153254711655328No Hit
CTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACGGCGCCGGAGGCACGACCCGGCCAGTTAAGGCCAGGAGCGC248780.11474270845555706No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG240520.11093301807914857No Hit
CTTTGAGCACTCTAATTTCTTCAAAGTAACGGCGCCGGAGGCACGACCCGGCCAGTTAAGGCCAGGAGCGCATCG235220.10848854362455235No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCT35700.047.7332539
ACGTCTG48550.042.49142515
TCGTATG43750.042.26384745
CTCGTAT40600.042.2287944
TTCCGTA48250.037.81983636
CACGTCT55500.037.41940314
TATGCCG51750.035.5971548
CGTATGC55600.033.31852746
AGTCACA59900.032.30690428
TATCTCG53650.031.8275941
ACACGTC67950.031.42660913
GTCACAG62150.030.85978529
CAGTCAC64500.029.89588427
GTATGCC64950.028.62832647
AGTTCCG67050.027.93587734
CTATAAG98650.027.31116746
TTACCTC42700.026.90330915
ATGCCGT70400.026.85308649
GTCTGAA79550.026.40841917
TCCGTAT70100.026.22832137
Produced by FastQC (version 0.11.2)