FastQCFastQC Report
Mon 3 Apr 2017
HGV7YBGX2_n01_9w-44_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGV7YBGX2_n01_9w-44_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35173520
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG443470.12608064248332268No Hit
CTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACGGCGCCGGAGGCACGACCCGGCCAGTTAAGGCCAGGAGCGC368590.10479190027043071No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG67000.036.96861615
CTCGTAT51850.036.1916542
TCGTATG62500.034.66103743
CACGTCT73950.033.867714
TATGCCG68600.031.52999946
CGTATGC74300.029.34299544
TTACCTC76900.028.66540715
ACACGTC88800.028.28179213
TGTTACC81050.027.45334213
CGCTATG96350.026.70824429
CAGTCAC86350.026.2059427
CTATAAG139250.025.9377346
TAGTTGT90500.025.653349
TCTAGTT102200.025.5169737
GTCGCTA101700.025.40499727
CTAATGC86900.025.2458921
GTATGCC89450.024.91334545
TATAAGC150200.024.13866647
CTTAAAC97350.023.9239851
ACATCCG151900.023.8740555
Produced by FastQC (version 0.11.2)