FastQCFastQC Report
Mon 3 Apr 2017
HGV7YBGX2_n01_9w-40_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGV7YBGX2_n01_9w-40_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6913584
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG149010.2155322044253747RNA PCR Primer, Index 33 (100% over 43bp)
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG88340.12777743063510907No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG71010.1027108370998313No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG26950.057.4709543
TATGCCG26950.057.21743846
CTCGTAT23350.056.13726442
ACGTCTG30600.052.42284815
CGTATGC30400.050.72364444
CACGTCT31950.050.3172314
CAGTCAC32050.049.29376627
GTATGCC31600.048.79778345
ATGCCGT31200.048.76035347
ACACGTC33000.048.7172813
AGTCACC34600.045.76053628
GCCGTCT35200.043.6117349
CCGTCTT35250.043.5498750
GTCTGAA37100.043.2351317
TGCCGTC38950.040.03288748
AGCACAC42500.038.1525110
CCAGTCA42450.037.4608626
CTCCAGT43300.036.9644824
GCTTGAA43900.035.92664759
GAACTCC45300.035.48453521
Produced by FastQC (version 0.11.2)