FastQCFastQC Report
Mon 3 Apr 2017
HGV7YBGX2_n01_9s-59_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGV7YBGX2_n01_9s-59_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20478775
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG310230.15148855339247586No Hit
CTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACGGCGCCGGAGGCACGACCCGGCCAGTTAAGGCCAGGAGCGC284650.13899757187624748No Hit
CTTTGAGCACTCTAATTTCTTCAAAGTAACGGCGCCGGAGGCACGACCCGGCCAGTTAAGGCCAGGAGCGCATCG243310.11881081754157659No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG236180.11532916397587259No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG29650.038.1601443
CTCGTAT28000.035.8501142
ACGTCTG38450.034.63146215
TATGCCG34900.033.40950846
CACGTCT43550.030.89371314
CGTATGC38850.029.30194344
CTTAAAC47900.028.596741
CTATAAG88900.028.32695646
CGCTATG47850.027.53808429
TATAAGC94550.026.70726647
CAGTCAC48000.025.94288627
GTCGCTA50650.025.74332627
ACACGTC54050.025.59449613
ACCTATA52750.025.1767166
ACATCCG101850.024.90098455
GAGTCCT104250.024.35332741
GTATGCC47500.024.25652145
GATCCCT104800.023.9129564
CCCTGGT105050.023.6915767
TAAACCT58150.023.669283
Produced by FastQC (version 0.11.2)