FastQCFastQC Report
Mon 3 Apr 2017
HGV7YBGX2_n01_9s-53_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGV7YBGX2_n01_9s-53_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19425182
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC215200.11078403280854716No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG204590.10532205052184325TruSeq Adapter, Index 2 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT43700.041.67917642
TCGTATG47350.040.360843
ACGTCTG53300.038.9629315
TATGCCG52400.036.93303746
TATCTCG49050.036.7814939
CGTATGC57800.033.1838744
CACGTCT64350.032.91605414
ACACGTC66350.031.50799213
CAGTCAC66850.030.03219627
GTATGCC65550.029.68162245
ATGCCGT69500.028.19336947
CACCGAT70850.027.70335631
TGTTACC53950.026.60056713
CACACGT78500.026.54352212
TTACCTC54950.026.17885615
AGTCACC79650.025.07600428
CGATGTA78300.024.80332634
TCTAGTT65950.024.6358937
TGTATCT75000.024.56084637
GTCTGAA86100.024.51949917
Produced by FastQC (version 0.11.2)