FastQCFastQC Report
Mon 3 Apr 2017
HGV7YBGX2_n01_9s-51_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGV7YBGX2_n01_9s-51_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13222555
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACGGCGCCGGAGGCACGACCCGGCCAGTTAAGGCCAGGAGCGC173780.13142694433866978No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG163550.12369016426855475No Hit
CTTTGAGCACTCTAATTTCTTCAAAGTAACGGCGCCGGAGGCACGACCCGGCCAGTTAAGGCCAGGAGCGCATCG156600.11843399403519213No Hit
CGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTAATTCTCCGT140500.10625782989747443No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG26350.034.3006315
CTCGTAT19000.033.04262542
TCGTATG22450.033.03572543
TATGCCG24550.030.6327846
GTCACAC25650.030.25916129
CGTATGC24850.029.2908944
CACGTCT31700.028.83841314
CTTAAAC37900.027.3109861
ACACGTC34000.027.1921713
ACCTATA39550.025.9044866
CGCTATG25150.024.41426829
AGTCACA33500.024.19829428
TTAAACC43650.024.1045282
CACAGTG33450.023.09982533
CAGTCAC35000.023.06266827
CACACAG33800.022.86062631
CTCCGTA45500.022.29037346
TAAACCT47900.021.8923913
GTATGCC34700.021.6723945
AACCTAT49000.021.6125325
Produced by FastQC (version 0.11.2)